Materials+and+Procedure

-//Computer Materials//: • CO2 Probe (3) • LABPRO (1) • Rubber Stopper (3) • 250 mL Flasks (3) • Yeast (7g per flask; 21g total) • Glucose (15g total) • 37° Water (100 mL per flask; 300 mL total) • Marker (1) • 100 mL Graduated Cylinder (3) • Scale (1) • Incubator (1) • Petri Dish (3)
 * Materials and Procedure**

-//Computer Procedure//: 1. Gather materials 2. Plug all LABPRO equipment into computer and configure software. 3. Plug 3 CO2 Probes into LABPRO and wait 2 minutes for it to stabilize at around 400 ppm 4. Put rubber stoppers over the base of all three CO2 Probes 5. Using a scale, measure 7g of yeast into 3 Petri dishes 6. Incubate Petri dishes for 10 minutes 7. Fill 3 100 mL graduated cylinders with 100 mL of 37° water 8. Take 3 250 mL flasks and label them “0”, “5”, and “10” with a marker 9. In each flask, pour 100 mL of water and 7g of yeast 10. Using the scale, measure 5 grams of glucose. Put this into the flask labeled “5” 11. Using the scale, measure 10 grams of glucose. Put this into the flask labeled “10” 12. Insert the 3 CO2 Probes into the 3 flasks. 13. Collect data for 5 minutes, with 4 samples per minute. 14. Remove probes, disconnect all devices, and clean up lab station.

-//Non-Computer Materials://
 * Yeast (21g total, 7g per flask)
 * Flasks (3)
 * Incubator (1)
 * Graduated cylinders (3)
 * Sugar (15g total)
 * Pipettes (3)
 * Water at 37 degrees C (300 mL total, 100 mL per flask)
 * Test tubes (3)
 * Stand (3)
 * Scale (1)
 * Marker (1)
 * Petri dishes (3)
 * Beakers (3)

-//Non-Computer Procedure://
 * 1)  Gather materials.
 * 2)  Label the three 250 mL flasks– 0, 5 and 10 grams of sugar
 * 3)  Use the scale to measure 7 grams of yeast and put them in three Petri dishes.
 * 4)  Let them incubate for 10 minutes.
 * 5)  Pour each Petri dish containing 7 grams of yeast into one of the three flasks.
 * 6)  Put 0, 5 or 10 grams of sugar in the correctly labeled flasks.
 * 7)  Put 100 mL of water (at 37 degrees C) in each flask.
 * 8)  Take three test tubes and label them 0, 5 and 10 grams of sugar.
 * 9)  Take three pipettes and fill each one with a sample of the 0, 5 or 10 gram sugar solution.
 * 10)  Fill up the test tubes with water and put the pipettes in them, making sure to keep the labels correct.
 * 11)  Fill up three beakers with water and put the test tubes in them
 * 12)  Get three stands to hold the test tubes.
 * 13)  Collect data for the volume of water displaced in mL every minute from all three beakers for a span of 10 minutes.
 * 14)  Clean up lab station.
 * 15)  Analyze the data