Microscopes

=__Types of Microscopes and Their Functions__=

Parts of a microscope:

 * Ocular Lens (Eyepiece)-look though it to see the specimen
 * Coarse Focus or Coarse Adjustment Knob- makes "big-scale" adjustments in order to see the specimen clearly
 * Fine Focus or Fine Adjustment Knob- makes "small-scale" adjustments in order to see the specimen clearly
 * Arm- holds the lenses in place
 * Base- holds the microscope
 * Illuminator- light to see the specimen
 * Stage- holds the specimen
 * Stage Clamps- hold specimen on the stage
 * Objective Lenses- magnification lens
 * Revolving Nosepiece- allows adjustment of magnification
 * Diaphragm- allows adjustment of amount of light that passes through

http://www.williamsclass.com/Microscope%20use/Images/JPG%20Images/MicroscopeLabeled.gif

Microscopes

 * Name || Preparation Time || Alive/Dead || View || Magnification ||
 * Scanning Electron Microscope (SEM) || 1-2 hours || Dead || 3D || 0x-10,000x ||
 * Transmision Electron Microscope (TEM) || 1-2 days || Dead || 2D || 0x- 100,000x ||
 * Light Microscope || 1-2 minutes || Alive/ Dead || 2D || less then 1,000x ||

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 * Light Microscope:**

Uses light and lenses to magnify specimen. Specimens can be alive or dead. 2D images.

http://www.southwestschools.org/jsfaculty/Microscopes/images/LightMicroscope.GIF

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 * Scanning Electron Microscope (SEM):**

This microscope has a 3D view and uses electrons to see features of a specimen. An “electron gun” in the microscope “fires” electrons onto the gold or other metal-coated object, which bounce off and hit sensors. This produces an image of the surface of the specimen. The specimen must be dead in order to be looked at, and it takes 1-2 hours to prepare. The microscope has a magnification ranging from 1,000-10,000x.

http://www.uq.edu.au/nanoworld/images/equip/6400.jpg

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 * Transmission Electron Microscope (TEM):**

Electronic beams are transmitted through a thin cross section of a specimen and detected by sensors on the other side, making an image**.** It takes 1-2 days to prepare, the specimen must be dead, and is used to see features of the surface, but in 2D. It has a magnification up to 1,000,000x! http://www.rsic.puchd.ac.in/ images/image002.jpg